We explore vitrification, a method that uses high concentrations of chemicals called cryoprotective agents (CPAs) to preserve organs at very low temperatures without ice formation. While this method could theoretically keep organs viable indefinitely, the high CPA concentrations needed can be toxic to tissues. Our study tested three CPAs—VM3, M22-PVP and M22—on rat kidney slices to assess their toxicity. We found that VM3 was the least toxic, followed by M22-PVP and M22. These findings highlight the importance of selecting CPAs that are less toxic for organ preservation. Our results, combined with future studies on how temperature affects CPA toxicity, will help develop better methods for long-term kidney storage. This could significantly improve organ transplantation by increasing available organs, enhancing donor-recipient matching and promoting fair access, ultimately improving patient outcomes.

When it comes to the medical field, 3D modeling has previously been used to render anatomical images in greater detail in order to better understand bodily functions. Lately, however, 3D modeling has made waves in depicting diseases, with a focus on their severity and progression. Unlike a model depicting computer graphics, 3D culture models allow cells to interact in three dimensions and better display cell growth and movement, according to the Food and Drug Administration. Culture models are beneficial in replicating the complexities of disease by promoting interactions between cells and providing insight into potential solutions. In this issue of the Journal of Young Investigators, Priscilla Detwieler and her colleagues demonstrate that atelocollagen incorporated in a 3D model is shown to simulate a potential treatment for inflammation-induced osteoarthritis.  

To combat the harmful effects of stress, neuroscientists are pointing to mindfulness, defined as the practice of being fully present and aware of our external environment and our actions, while not being overly reactive or overwhelmed by external events. To shed light on this, JYI interviewed renowned neuroscientist Dr. Alexandra Fiocco, whose expertise lies at the intersection of mindfulness, stress, and cognitive aging. Dr. Fiocco currently does research at Stress and Healthy Aging Research (StAR) Lab and teaches at Toronto Metropolitan University.